MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.Report as inadecuate




MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Interfaces biomatériaux-tissus hôtes 2 LIB - Laboratoire d-Immunologie et de Biotechnologies 3 MéDIAN - Médicaments : Dynamique Intracellulaire et Architecture Nucléaire 4 LPC - Laboratoire de Physique Corpusculaire - Clermont-Ferrand 5 GAILLARD - LE GAILLARD

Abstract : After calcium-phosphates biomaterials based implantation like hydroxyapatite HA coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases MMPs, which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases TIMPs are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics size, shape and sintering temperature on the MMP-2 -9 and their respective inhibitors TIMP-2 -1 production. Our results demonstrate that sintering temperature that modify crystal size and surface area have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1β release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1β.

Keywords : Matrix metalloproteinase Tissue inhibitors of metalloproteinase Hydroxyapatite particles Human monocytes Physical characteristics





Author: Patrice Laquerriere - Alexia Grandjean-Laquerriere - Salima Addadi-Rebbah - Edouard Jallot - Dominique Laurent-Maquin - Patrick F

Source: https://hal.archives-ouvertes.fr/



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