RP-HPLC Method for Determination of Several NSAIDs and Their Combination DrugsReport as inadecuate




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Chromatography Research InternationalVolume 2013 2013, Article ID 242868, 13 pages

Research ArticleDepartment of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research NIPER, Balanagar, Hyderabad, Andhra Pradesh 500037, India

Received 29 June 2013; Accepted 13 October 2013

Academic Editor: Andrew Shalliker

Copyright © 2013 Prinesh N. Patel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An RP-HPLC method for simultaneous determination of 9 NSAIDs paracetamol, salicylic acid, ibuprofen, naproxen, aceclofenac, diclofenac, ketorolac, etoricoxib, and aspirin and their commonly prescribed combination drugs thiocolchicoside, moxifloxacin, clopidogrel, chlorpheniramine maleate, dextromethorphan, and domperidone was established. The separation was performed on Kromasil C18 250 × 4.6 mm, 5 μm at 35°C using 15 mM phosphate buffer pH 3.25 and acetonitrile with gradient elution at a flow rate of 1.1 mL-min. The detection was performed by a diode array detector DAD at 230 nm with total run time of 30 min. Calibration curves were linear with correlation coefficients of determination . Limit of detection LOD and Limit of quantification LOQ ranged from 0.04 to 0.97 μg-mL and from 0.64 to 3.24 μg-mL, respectively. As an application tool of quality by design, full factorial experimental design was used for the testing of robustness of the method. The prediction profiler correlating various parameters and responses was established from the results of design of experiments DOE.





Author: Prinesh N. Patel, Gananadhamu Samanthula, Vishalkumar Shrigod, Sudipkumar C. Modh, and Jainishkumar R. Chaudhari

Source: https://www.hindawi.com/



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