Denoising of high-resolution single-particle electron-microscopy density maps by their approximation using three-dimensional Gaussian functionsReport as inadecuate




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* Corresponding author 1 IMPMC - Institut de minéralogie, de physique des matériaux et de cosmochimie 2 Biocomputing Unit Madrid

Abstract : Cryo-electron microscopy cryo-EM of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are currently being obtained at subnanometer resolution but the interpretation of structural details in such EM-derived maps is often difficult because of noise at these high-frequency signal components that reduces their contrast. In this paper, we show that the method for EM density-map approximation using Gaussian functions can be used for denoising of single-particle EM maps of high typically subnanometer resolution. We show its denoising performance using simulated and experimental EM density maps of several complexes.

Keywords : Electron microscopy density map single particle analysis denoising macromolecular complexes structure Gaussian functions





Author: S. Jonić - J. Vargas - R. Melero - J. Gómez-Blanco - J.M. Carazo - C.O.S. Sorzano -

Source: https://hal.archives-ouvertes.fr/



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