Combined Analysis of EPHX1, GSTP1, GSTM1 and GSTT1 Gene Polymorphisms in Relation to Chronic Obstructive Pulmonary Disease Risk and Lung Function ImpairmentReport as inadecuate




Combined Analysis of EPHX1, GSTP1, GSTM1 and GSTT1 Gene Polymorphisms in Relation to Chronic Obstructive Pulmonary Disease Risk and Lung Function Impairment - Download this document for free, or read online. Document in PDF available to download.

Disease Markers - Volume 30 2011, Issue 5, Pages 253-263



Biochemistry and Molecular Biology Laboratory, Faculty of Pharmacy, Monastir, Tunisia

Pulmonology Department, CHU Tahar Sfar, Mahdia, Tunisia

Haematology Laboratory, Faculty of Pharmacy and CHU Fattouma Bourguiba, Monastir, Tunisia

Institute of Human Genetics and Montpellier 2 University, Montpellier, France

Received 28 June 2011; Accepted 28 June 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Smoking is considered as the major causal factor of chronic obstructive pulmonary disease COPD. Nevertheless, a minority of chronic heavy cigarette smokers develops COPD. This suggests important contribution of other factors such as genetic predisposing. Our objective was to investigate combined role of EPHX1, GSTP1, M1 and T1 gene polymorphisms in COPD risk, its phenotypes and lung function impairment. Prevalence of EPHX1, GSTP1, M1 and T1 gene polymorphisms were assessed in 234 COPD patients and 182 healthy controls from Tunisia. Genotypes of EPHX1 Tyr113His; His139Arg and GSTP1 Ile105Val; Ala114Val polymorphisms were performed by PCR-RFLP, while the deletion in GSTM1 and GSTT1 genes was determined using multiplex PCR. Analysis of combinations showed a significant association of 113His-His EPHX1-null-GSTM1 OR = 4.07 and null-GSTM1-105Val-Val GSTP1 OR = 3.56 genotypes with increased risk of COPD respectively P=0.0094 and P=0.0153. The null-GSTM1- null-GSTT1, 105Val-Val GSTP1-null GSTT1, 113His-His EPHX1-null-GSTM1 and null-GSTM1-105Val-Val GSTP1 genotypes were related to emphysema respectively P = 0.01; P = 0.009; P = 0.008 and P = 0.001. Combination of 113His-His EPHX1-null-GSTM1 genotypes showed a significant association with the decrease of ΔFEV1 in patients P = 0.028.

In conclusion, our results suggest combined EPHX1, GSTP1, GSTM1 and GSTT1 genetic polymorphisms may play a significant role in the development of COPD, emphysema and decline of the lung function.





Author: Ramzi Lakhdar, Sabri Denden, Jalel Knani, Nadia Leban, Houria Daimi, Mohsen Hassine, Gérard Lefranc, Jemni Ben Chibani, and Amel

Source: https://www.hindawi.com/



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