Standardised Models for Inducing Experimental Peritoneal Adhesions in Female RatsReport as inadecuate

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BioMed Research International - Volume 2014 2014, Article ID 435056, 8 pages -

Research Article

University Hospital for Women, University of Tuebingen, Calwerstraße 4, 72076 Tuebingen, Germany

Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA

Department of Pathology, University Hospital Mainz, Langenbeckstraße 1, 69120 Heidelberg, Germany

University Hospital for Women, Im Neuenheimer Feld 440, 69120 Heidelberg, Germany

Received 24 February 2014; Accepted 19 March 2014; Published 8 April 2014

Academic Editor: Geoffrey Trew

Copyright © 2014 Bernhard Kraemer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns group 1, brushing of parietal peritoneum only group 2, sharp excision of parietal peritoneum closed with interrupted sutures group 3, ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture group 4, bipolar electrocoagulation of the peritoneum group 5, and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures group 6. Upon second look, there were significant differences in the adhesion incidence between the groups . Analysis of the fraction of adhesions showed that groups 2 0% and 5 4% were significantly less than the other groups . Furthermore, group 6 69% was significantly higher than group 1 48% and group 4 47% . There was no difference between group 3 60% and group 6 . From a clinical viewpoint, comparison of different electrocoagulation modes and pharmaceutical adhesion barriers is possible with standardised models.

Author: Bernhard Kraemer, Christian Wallwiener, Taufiek K. Rajab, Christoph Brochhausen, Markus Wallwiener, and Ralf Rothmund



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