Lespedeza davurica Lax. Schindl. Extract Protects against Cytokine-Induced β-Cell Damage and Streptozotocin-Induced DiabetesReport as inadecuate




Lespedeza davurica Lax. Schindl. Extract Protects against Cytokine-Induced β-Cell Damage and Streptozotocin-Induced Diabetes - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2015 2015, Article ID 169256, 8 pages -

Research Article

Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, College of Natural Science, Mokpo National University, 1666 Youngsan-ro, Muan-gun, Jeonnam 534-729, Republic of Korea

Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA

Received 10 July 2014; Revised 5 December 2014; Accepted 16 December 2014

Academic Editor: Gabriel F. Anhe

Copyright © 2015 Bhesh Raj Sharma and Dong Young Rhyu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lespedeza has been used for the management of diabetes in folklore medicine. The purpose of this study is to investigate the protective effects of the methanol extract of Lespedeza davurica LD on cytokine-induced β-cell damage and streptozotocin- STZ- induced diabetes. RINm5F cells were treated with interleukin- IL- 1β and interferon- IFN- γ to induce pancreatic β-cell damage. The exposure of LD extract significantly decreased cell death, nitric oxide NO production, nitric oxide synthase iNOS expression, and nucleus factor-kappa B NF-κB p65 activation. Antidiabetic effects of LD extract were observed by oral glucose tolerance test OGTT in normal rats and by checking the biochemical, physiological, and histopathological parameters in STZ-induced diabetic rats. In OGTT, glucose clearance levels improved by oral treatment of LD extract. The water intake, urine volume, blood glucose, and serum TG, TC, TBARS, and DPP-IV levels were significantly decreased, and liver glycogen content was significantly increased by treatment of LD extract 250 mg-kg BW in STZ-induced diabetic rats. Also, insulin immunoreactivity of the pancreases was increased in LD extract administrated rats compared with diabetic control rats. These results indicate that LD extract may protect pancreatic β-cell damage and regulate the blood glucose in STZ-induced diabetic rats.





Author: Bhesh Raj Sharma and Dong Young Rhyu

Source: https://www.hindawi.com/



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