Survival of honey bee Apis mellifera pupae after trypan blue staining of wounds caused by Varroa destructor mites or artificial perforationReport as inadecuate




Survival of honey bee Apis mellifera pupae after trypan blue staining of wounds caused by Varroa destructor mites or artificial perforation - Download this document for free, or read online. Document in PDF available to download.



Abstract : The recently described technique of vital staining with trypan blue to visualise pupal wounds of honey bees, originating from punctures made by Varroa destructor mites, was applied to artificial perforations performed with a fine needle. The stained pupae were subsequently reared in vitro until eclosion of the adult bees. Their mortality was recorded daily. The survival of the treated pupae was only moderately affected by the staining procedure. No obvious toxic effects caused by the compound trypan blue were observed. The process of wound healing was normal. Pupae with previously trypan blue stained integumental wounds can therefore be used for long lasting experiments. This is of particular interest for future studies on the assumed vector function of Varroa mites in the transfer of bacterial and viral pathogens in honey bee diseases.

Keywords : Apis mellifera Varroa destructor pupal wounds vital staining trypan blue survival





Author: Matthias Herrmann Ghazwan Kanbar Wolf Engels

Source: https://hal.archives-ouvertes.fr/



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