Increased DNA Dicarbonyl Glycation and Oxidation Markers in Patients with Type 2 Diabetes and Link to Diabetic NephropathyReport as inadecuate




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Journal of Diabetes Research - Volume 2015 2015, Article ID 915486, 10 pages -

Research Article

Warwick Medical School, Clinical Sciences Research Laboratories, University of Warwick, University Hospital, Coventry CV2 2DX, UK

Human Nutrition & Metabolism Research and Training Center Graz, Institute of Molecular Biosciences, Karl Franzens University, 8010 Graz, Austria

Clinical Division of Nephrology, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria

Clinical Division of Endocrinology and Nuclear Medicine, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria

Warwick Systems Biology Centre, Senate House, University of Warwick, Coventry CV4 7AL, UK

Received 1 January 2015; Revised 14 March 2015; Accepted 23 March 2015

Academic Editor: Patrizio Tatti

Copyright © 2015 Sahar Waris et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. The aim of this study was to assess the changes of markers of DNA damage by glycation and oxidation in patients with type 2 diabetes and the association with diabetic nephropathy. Methodology. DNA oxidation and glycation adducts were analysed in plasma and urine by stable isotopic dilution analysis liquid chromatography-tandem mass spectrometry. DNA markers analysed were as follows: the oxidation adduct 7,8-dihydro-8-oxo-2′-deoxyguanosine 8-OxodG and glycation adducts of glyoxal and methylglyoxal—imidazopurinones GdG, MGdG, and N2-1,R-S-carboxyethyldeoxyguanosine CEdG. Results. Plasma 8-OxodG and GdG were increased 2-fold and 6-fold, respectively, in patients with type 2 diabetes, with respect to healthy volunteers. Median urinary excretion rates of 8-OxodG, GdG, MGdG, and CEdG were increased 28-fold, 10-fold, 2-fold, and 2-fold, respectively, in patients with type 2 diabetes with respect to healthy controls. In patients with type 2 diabetes, nephropathy was associated with increased plasma 8-OxodG and increased urinary GdG and CEdG. In a multiple logistic regression model for diabetic nephropathy, diabetic nephropathy was linked to systolic blood pressure and urinary CEdG. Conclusion. DNA oxidative and glycation damage-derived nucleoside adducts are increased in plasma and urine of patients with type 2 diabetes and further increased in patients with diabetic nephropathy.





Author: Sahar Waris, Brigitte M. Winklhofer-Roob, Johannes M. Roob, Sebastian Fuchs, Harald Sourij, Naila Rabbani, and Paul J. Thorn

Source: https://www.hindawi.com/



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