Structure of artificial and natural VE-cadherin-based adherens junctions.Report as inadecuate

Structure of artificial and natural VE-cadherin-based adherens junctions. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 CBMN - Chimie et Biologie des Membranes et des Nanoobjets 2 Physiopathologie vasculaire : interactions cellulaires, signalisation et vieillissement 3 IBS - UMR 5075 - Institut de biologie structurale

Abstract : In vascular endothelium, adherens junctions between endothelial cells are composed of VE-cadherin vascular endothelial cadherin, an adhesive receptor that is crucial for the proper assembly of vascular structures and the maintenance of vascular integrity. As a classical cadherin, VE-cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Although, from structural crystallographic data, a dimeric structure arranged in a trans orientation has emerged as a potential mechanism of cell-cell adhesion, the cadherin organization within adherens junctions remains controversial. Concerning VE-cadherin, its extracellular part possesses the capacity to self-associate in solution as hexamers consisting of three antiparallel cadherin dimers. VE-cadherin-based adherens junctions were reconstituted in vitro by assembly of a VE-cadherin EC extracellular repeat 1-EC4 hexamer at the surfaces of liposomes. The artificial adherens junctions revealed by cryoelectron microscopy appear as a two-dimensional self-assembly of hexameric structures. This cadherin organization is reminiscent of that found in native desmosomal junctions. Further structural studies performed on native VE-cadherin junctions would provide a better understanding of the cadherin organization within adherens junctions. Homophilic interactions between cadherins are strengthened intracellularly by connection to the actin cytoskeleton. Recently, we have discovered that annexin 2, an actin-binding protein connects the VE-cadherin-catenin complex to the actin cytoskeleton. This novel link is labile and promotes the endothelial cell switch from a quiescent to an angiogenic state.

Keywords : Cryoelectron microscopy VE cadherin 3D reconstruction endothelial junction annexin 2

Author: Jean-Christophe Taveau - Mathilde Dubois - Olivier Le Bihan - Sylvain Trépout - Sébastien Almagro - Elizabeth Hewat - Claire Du



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