The Association of High Prevalence of Trophozoites in Peripheral Blood with Lower Antibody Response to P. falciparum Infected Erythrocytes among Asymptomatic Children in SudanReport as inadecuate




The Association of High Prevalence of Trophozoites in Peripheral Blood with Lower Antibody Response to P. falciparum Infected Erythrocytes among Asymptomatic Children in Sudan - Download this document for free, or read online. Document in PDF available to download.

Mediators of Inflammation - Volume 2016 2016, Article ID 7987686, 4 pages -

Research Article

Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan

Faculty of Medicine, Al-Zaiem Al Azhari University, Khartoum, Sudan

Institute of Endemic Diseases, University of Khartoum, 11111 Khartoum, Sudan

The Blue Nile Institute for Training and Research, Gezira University, Wad Madani, Sudan

College of Pharmacy, The National Ribat University, Khartoum, Sudan

Received 12 March 2016; Accepted 1 June 2016

Academic Editor: Christophe Chevillard

Copyright © 2016 Sara N. Mohamed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The most prominent variant surface antigens VSAs of Plasmodium falciparum are the var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 PfEMP1 family, which serves as a parasite-sequestering ligand to endothelial cells. In this study we have examined the antibody reactivity of autologous plasma from symptomatic and asymptomatic malaria infected children against the infected erythrocytes’ surface antigens using flow cytometry. Methods. Ethidium-bromide-labelled erythrocytic mature forms of P. falciparum parasites obtained from symptomatic and asymptomatic children were sequentially incubated with autologous plasma and fluorescein isothiocyanate-conjugated FITC antihuman IgG. Plasma antibody reactivity was detected by flow cytometry. Results. Asymptomatic children had more prevalence of trophozoites in peripheral blood 66% compared to symptomatic children 16%, . The mean percentage of infected RBCs reacting with autologous sera was 89.78 among symptomatic children compared to 79.62 among asymptomatic children . Moreover, the mean fluorescence intensity MFI in the asymptomatic was significantly higher compared to symptomatic children value = 0.040. Conclusion. Variant surface antigens on Plasmodium falciparum infected RBCs from symptomatic malaria children tend to be better recognized by IgG antibodies. This may suggest a role of some IgG antibodies in severity of malaria.





Author: Sara N. Mohamed, Dina A. Hassan, Abdelrahim M. El Hussein, Ihssan M. Osman, Muntasir E. Ibrahim, Hiba S. Mohamed, Bakri Y. N

Source: https://www.hindawi.com/



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