Functional Recovery after Scutellarin Treatment in Transient Cerebral Ischemic Rats: A Pilot Study with 18F-Fluorodeoxyglucose MicroPETReport as inadecuate




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Evidence-Based Complementary and Alternative MedicineVolume 2013 2013, Article ID 507091, 9 pages

Research Article

Department of Nuclear Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China

Department of Traditional Chinese Medicine & Rehabilitation, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China

Zhejiang University Medical PET Center, Hangzhou, Zhejiang 310009, China

Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, Zhejiang 310009, China

Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, Zhejiang 310009, China

Department of Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China

Received 28 February 2013; Accepted 12 April 2013

Academic Editor: Mei Tian

Copyright © 2013 Jin-hui Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate neuroprotective effects of scutellarin Scu in a rat model of cerebral ischemia with use of

F-fluorodeoxyglucose

F-FDG micro positron emission tomography microPET. Method. Middle cerebral artery occlusion was used to establish cerebral ischemia. Rats were divided into 5 groups: sham operation, cerebral ischemia-reperfusion untreated CIRU group, Scu-25 group Scu 25 mg-kg-d, Scu-50 group Scu 50 mg-kg-d, and nimodipine 10 mg-Kg-d. The treatment groups were given for 2 weeks. The therapeutic effects in terms of cerebral infarct volume, neurological deficit scores, and cerebral glucose metabolism were evaluated. Levels of vascular density factor vWF, glial marker GFAP, and mature neuronal marker NeuN were assessed by immunohistochemistry. Results. The neurological deficit scores were significantly decreased in the Scu-50 group compared to the CIRU group .

F-FDG accumulation in the ipsilateral cerebral infarction increased steadily over time in Scu-50 group compared with CIRU group and Scu-25 group . Immunohistochemical analysis demonstrated Scu-50 enhanced neuronal maturation. Conclusion.

F-FDG microPET imaging demonstrated metabolic recovery after Scu-50 treatment in the rat model of cerebral ischemia. The neuroprotective effects of Scu on cerebral ischemic injury might be associated with increased regional glucose activity and neuronal maturation.





Author: Jin-hui Li, Jing Lu, and Hong Zhang

Source: https://www.hindawi.com/



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