Scutellarin Reduces Endothelium Dysfunction through the PKG-I PathwayReport as inadecuate




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Evidence-Based Complementary and Alternative Medicine - Volume 2015 2015, Article ID 430271, 12 pages -

Research Article

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China

Department of Respiratory Medicine, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

Pharmacy Department, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

Department of Clinical Pharmacy, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

Received 4 May 2015; Revised 25 July 2015; Accepted 4 August 2015

Academic Editor: Dan Hu

Copyright © 2015 Xiaohua Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. In this report, we investigated the protective mechanism of scutellarin SCU in vitro and in vivo which could be involved in endothelial cGMP-dependent protein kinase PKG, vasodilator stimulated phosphoprotein VASP pathway, and vascular endothelium dysfunction EtD. Method. Human brain microvascular endothelial cells HBMECs with hypoxia reoxygenation HR treatment and rats with cerebral ischemia reperfusion CIR treatment were applied. Protein and mRNA expression of PKG, VASP, and p-VASP were evaluated by Western blot and RT-PCR methods. Vascular EtD was assessed by using wire myography to determine endothelium-dependent vasorelaxation in isolated rat basilar artery BA. Result. In cultured HBMECs, SCU 0.1, 1, and 10 μM increased cell viability, mRNA, protein level, and phosphorylative activity of PKG and VASP against HR injury. In HR model of BA, SCU increased protein level of P-VASP. In rat CIR model, wire myography demonstrated that SCU 45 and 90 mg-kg, i.v. significantly reduced ischemic size by partially restoring the endothelium dependent vasodilation of BA; PKG inhibitor Rp-8-Br-cGMPS 50 μg-kg, i.v. reversed this protection of SCU in CIR rats. Conclusion. SCU protects against cerebral vascular EtD through endothelial PKG pathway activation.





Author: Xiaohua Du, Chen Chen, Min Zhang, Donghua Cai, Jiaqi Sun, Jian Yang, Na Hu, Congji Ma, Liyan Zhang, Jun Zhang, and Weimi

Source: https://www.hindawi.com/



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