Polyphenol-Rich Extract from Propolis Reduces the Expression and Activity of Streptococcus mutans Glucosyltransferases at Subinhibitory ConcentrationsReport as inadecuate




Polyphenol-Rich Extract from Propolis Reduces the Expression and Activity of Streptococcus mutans Glucosyltransferases at Subinhibitory Concentrations - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2016 2016, Article ID 4302706, 7 pages -

Research Article

Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus BIOREN, Universidad de La Frontera, Avenida Francisco Salazar 01145, 4811230 Temuco, Chile

Departamento de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad San Sebastián, Campus Los Leones, Lota 2465, 7510157 Providencia, Santiago, Chile

Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile

Received 10 December 2015; Accepted 8 March 2016

Academic Editor: Kimon A. Karatzas

Copyright © 2016 Jorge Jesús Veloz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tooth decay is an infectious disease, whose main causative agent identified is Streptococcus mutans S. mutans. Diverse treatments have been used to eradicate this microorganism, including propolis. To date, it has been shown that polyphenols from Chilean propolis inhibit S. mutans growth and biofilm formation. However, the molecular mechanisms underlying this process are unclear. In the present study, we assessed the effect of Chilean propolis on the expression and activity of the glycosyltransferases enzymes and their related genes. Polyphenol-rich extract from propolis inhibited gene expression of glycosyltransferases GtfB, GtfC, and GtfD and their related regulatory genes, for example, VicK, VicR, and CcpA. Moreover, the treatment inhibited glucosyltransferases activity measured by the formation of sucrose-derived glucans. Additionally, an inhibitory effect was observed in the expression of SpaP involved in sucrose-independent virulence of S. mutans. In summary, our results suggest that Chilean propolis has a dose-dependent effect on the inhibition of genes involved in S. mutans virulence and adherence through the inhibition of glucosyltransferases, showing an anticariogenic potential of polyphenols from propolis beyond S. mutans growth inhibition.





Author: Jorge Jesús Veloz, Nicolás Saavedra, Marysol Alvear, Tomás Zambrano, Leticia Barrientos, and Luis A. Salazar

Source: https://www.hindawi.com/



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