Development of Neurological Mouse Model for Toxoplasmosis Using Toxoplasma gondii Isolated from Chicken in KenyaReport as inadecuate




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Pathology Research International - Volume 2017 2017, Article ID 4302459, 8 pages - https:-doi.org-10.1155-2017-4302459

Research Article

Department of Medical Laboratory Science, School of Medicine and Health Sciences, Kenya Methodist University, P.O. Box 45240-00100, Nairobi, Kenya

Department of Public Health, Jomo Kenyatta University of Agriculture and Technology JKUAT, P.O. Box 62000-00200, Nairobi, Kenya

Department of Animal Sciences, Jomo Kenyatta University of Agriculture and Technology JKUAT, P.O. Box 62000-00200, Nairobi, Kenya

Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology JKUAT, P.O. Box 62000-00200, Nairobi, Kenya

Department of Animal Health and Production, Mount Kenya University, P.O. Box 342-01000, Thika 342-01000, Kenya

Correspondence should be addressed to John Maina Kagira

Received 25 January 2017; Accepted 19 April 2017; Published 24 May 2017

Academic Editor: Shahid Pervez

Copyright © 2017 John Mokua Mose et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Animal models for the toxoplasmosis are scarce and have limitations. In this study, a neurological mouse model was developed in BALB-c mice infected intraperitoneally with 15 cysts of a Toxoplasma gondii isolate. The mice were monitored for 42 days and euthanized at different time points. Another group of mice were orally treated with dexamethasone DXM: 2.66 mg-kg daily, 5.32 mg-kg daily at 42 days after infection and monitored for a further 42 days. A mortality rate of 15% and 28.6% was observed in mice given 2.66 mg-kg-day and 5.32 mg-kg-day of DXM, respectively. The mean cyst numbers in the brain of DXM treated mice increased up to twofold compared with chronically infected untreated mice. Infections up to 42 days were associated with an increase in both IgM and IgG levels but following dexamethasone treatment, IgM levels declined but IgG levels continued on rising. The brain of toxoplasmosis infected mice showed mononuclear cellular infiltrations, neuronal necrosis, and cuffing. The severity of pathology was higher in mice treated with dexamethasone compared to the positive control groups. The findings of this study demonstrate that DXM-induced reactivation of chronic toxoplasmosis may be a useful development of laboratory animal model in outbred mice used for in vivo studies.





Author: John Mokua Mose, David Muchina Kamau, John Maina Kagira, Naomi Maina, Maina Ngotho, Adele Njuguna, and Simon Muturi Karanja

Source: https://www.hindawi.com/



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