Structure and stability of D-galactose-D-glucose-binding protein. The role of D-glucose binding and Ca ion depletionReport as inadecuate




Structure and stability of D-galactose-D-glucose-binding protein. The role of D-glucose binding and Ca ion depletion - Download this document for free, or read online. Document in PDF available to download.

Spectroscopy - Volume 24 2010, Issue 3-4, Pages 355-359



Institute of Cytology of the Russian Academy of Sciences, St. Petersburg, Russia

Institute of Protein Biochemistry CNR, Napoli, Italy

Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia



Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of guanidine hydrochloride GdnHCl on the structure and stability of the D-galactose-D-glucose-binding protein from Escherichia coli GGBP and its complex with D-glucose GGBP-Glc were investigated by intrinsic protein fluorescence and far-UV circular dichroism CD. The role of calcium in the stability of the protein structure was also studied. It was shown that the processes of GGBP and GGBP-Glc unfolding induced by GdnHCl followed one-step reversible denaturation mechanism. The obtained data showed that the binding of glucose to GGBP resulted in an increase of the protein stability towards the actions of the GdnHCl which made protein unfolding more cooperative. The stabilities of GGBP alone, GGBP in the presence of glucose, GGBP-depleted calcium GGBP-Ca, and GGBP-Glc-depleted calcium GGBP-Glc-Ca were characterized by difference of Gibbs free energies.





Author: Olga V. Stepanenko, Olga I. Povarova, Olesya V. Stepanenko, Alexander V. Fonin, Irina M. Kuznetsova, Konstantin K. Turoverov, Mar

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents