Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse ModelReport as inadecuate




Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse Model - Download this document for free, or read online. Document in PDF available to download.

Journal of Immunology Research - Volume 2017 2017, Article ID 9125048, 5 pages - https:-doi.org-10.1155-2017-9125048

Research Article

Research Center for Chemistry Indonesian Institute of Sciences, Kawasan Puspiptek Serpong, Tangerang, Indonesia

Eijkman Institute for Molecular Biology, Jakarta, Indonesia

Correspondence should be addressed to Dodi Safari

Received 17 February 2017; Revised 16 May 2017; Accepted 4 June 2017; Published 5 July 2017

Academic Editor: Kurt Blaser

Copyright © 2017 Agus Haryono et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. We investigate the immunogenic properties of chitosan and liposome nanoparticles as adjuvant codelivery against a commercial pneumococcal conjugate vaccine PCV in an animal model. Methods. The chitosan and liposome nanoparticles were prepared by ionic gelation and dry methods, respectively. The PCV immunization was performed intradermally in the presence of adjuvants and booster injections which were given without an adjuvant. The Quil-A® was used as a control adjuvant. The ELISA was performed to measure the antibodies against pneumococcal type 14 polysaccharide Pn14PS. Results. The level of total antibodies against Pn14PS antigen was no different between the mouse groups with or without adjuvant codelivery. Codelivery of the PCV with chitosan nanoparticles as well as the Quil-A adjuvant elicited IgG1, IgG2a, IgG2b, and IgG3 antibodies. Meanwhile, codelivery of liposome nanoparticles elicited mainly IgG1 antibodies against the Pn14PS. Conclusions. The chitosan and liposome nanoparticles as adjuvant codelivery were successfully synthesized. These nanoparticles have different shapes in particle formation, liposome nanoparticle with their unilamellar shape and chitosan nanoparticles in large shape due to the aggregation of small-size particles. Codelivery of chitosan nanoparticles has more effect on the IgG subclass antibody production than that of liposome nanoparticles in a mouse model.





Author: Agus Haryono, Korrie Salsabila, Witta Kartika Restu, Sri Budi Harmami, and Dodi Safari

Source: https://www.hindawi.com/



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