Effects of Palm Vitamin E on Bone-Formation-Related Gene Expression in Nicotine-Treated RatsReport as inadecuate




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Evidence-Based Complementary and Alternative MedicineVolume 2012 2012, Article ID 656025, 5 pages

Research Article

Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Received 29 May 2012; Revised 16 July 2012; Accepted 18 July 2012

Academic Editor: Ahmad Nazrun Shuid

Copyright © 2012 Seham Salem Ahmed Abukhadir et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The study determines the effects of palm vitamin E on the gene expression of bone-formation-related genes in nicotine-treated rats. Male rats were divided into three groups: normal saline olive oil NSO, nicotine olive oil NO, and nicotine palm vitamin E NE. The treatment was carried out in 2 phases. During the first 2 months, the NSO group received normal saline while the NO and NE groups received nicotine 7 mg-kg, 6 days a week, intraperitoneally. The following 2 months, normal saline and nicotine administration was stopped and was replaced with oral supplementation of olive oil for the NSO and NO groups and oral supplementation of palm vitamin E 60 mg-kg for the NE group. Both femurs were harvested to determine the gene expression of bone morphogenetic protein-2 BMP-2, Osterix OSX, and Runt-related transcription factor 2 RUNX2. Nicotine significantly downregulated the gene expression. This effect was reversed by palm vitamin E treatment. In conclusion, palm vitamin E may play a role in osteoblast differentiation and can be considered as an anabolic agent to treat nicotine-induced osteoporosis.





Author: Seham Salem Ahmed Abukhadir, Norazlina Mohamed, Suzana Makpol, and Norliza Muhammad

Source: https://www.hindawi.com/



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