Mouse Mesenchymal Progenitor Cells Expressing Adipogenic and Osteogenic Transcription Factors Suppress the Macrophage Inflammatory ResponseReport as inadecuate




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Stem Cells International - Volume 2017 2017, Article ID 5846257, 13 pages - https:-doi.org-10.1155-2017-5846257

Research Article

Department of Biology, Chemistry and Environmental Studies, Molloy College, 1000 Hempstead Avenue, Rockville Centre, NY 11570, USA

Biomedical Research Core, Winthrop University Hospital, 101 Mineola Blvd., Mineola, NY 11501, USA

Stony Brook University School of Medicine, Stony Brook, NY 11794, USA

Correspondence should be addressed to Jodi F. Evans

Received 3 September 2016; Revised 22 November 2016; Accepted 18 December 2016; Published 16 January 2017

Academic Editor: Armand Keating

Copyright © 2017 Natalie Fernandez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal progenitor cell characteristics that can identify progenitor populations with specific functions in immunity are actively being investigated. Progenitors from bone marrow and adipose tissue regulate the macrophage MΦ inflammatory response by promoting the switch from an inflammatory to an anti-inflammatory phenotype. Conversely, mesenchymal progenitors from the mouse aorta mAo support and contribute to the MΦ response under inflammatory conditions. We used cell lines with purported opposing immune-regulatory function, a bone marrow derived mesenchymal progenitor cell line D1 and a mouse aorta derived mesenchymal progenitor cell line mAo. Their interaction and regulation of the MΦ cell response to the inflammatory mediator, lipopolysaccharide LPS, was examined by coculture. As expected, D1 cells suppressed NO, TNF-α, and IL-12p70 production but MΦ phagocytic activity remained unchanged. The mAo cells enhanced NO and TNF-α production in coculture and enhanced MΦ phagocytic activity. Using flow cytometry and PCR array, we then sought to identify sets of MSC-associated genes and markers that are expressed by these progenitor populations. We have determined that immune-supportive mesenchymal progenitors highly express chondrogenic and tenogenic transcription factors while immunosuppressive mesenchymal progenitors highly express adipogenic and osteogenic transcription factors. These data will be useful for the isolation, purification, and modification of mesenchymal progenitors to be used in the treatment of inflammatory diseases.





Author: Natalie Fernandez, Heather Renna, Lauren McHugh, Katie Mazolkova, William Crugnola, and Jodi F. Evans

Source: https://www.hindawi.com/



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