Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella TyphimuriumReport as inadecuate




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ISRN Veterinary ScienceVolume 2012 2012, Article ID 512848, 7 pages

Research Article

Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture & Technology, Pantnagar 263145, India

Animal Biotechnology Center, Department of Veterinary Physiology & Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar 263145, India

Received 22 November 2011; Accepted 26 December 2011

Academic Editor: H. Akashi

Copyright © 2012 Richa Jha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Salmonella Typhimurium is an important pathogen having a broad host range. In human population it causes mostly gastroenteritis but there are reports in which it was found to be responsible to cause several lethal diseases like endocarditis and meningitis. Poultry products are the major sources of this organism in India as these are consumed at various stages of cooking. The available vaccines have their own limitations such as short-term immunity. Outer membrane proteins have shown some promising potential, so in the present study Omp C of Salmonella Typhimurium was cloned and sequenced to explore the possibility of development of r-DNA vaccine against Salmonella Typhimurium for poultry. The sequence of Omp C was studied for antigenic indexing, epitope mapping, and MHC mapping using various bioinformatic tools. The ORF analysis revealed a complete coding region of approximately 1000 bp. Five major and 13 minor B-cell epitopes were identified having an antigenic index of 1.7. The sequences also showed major histocompatibility complex MHC class I and class II binding region indicating a potential of eliciting cell-mediated immune response. The findings indicate that Omp C may be proven as promising candidate for development of r-DNA vaccine against Salmonella Typhimurium.





Author: Richa Jha, Anil Kumar, Anjani Saxena, Shantanu Tamuly, and M. K. Saxena

Source: https://www.hindawi.com/



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