Induction of the interleukin 6- signal transducer and activator of transcription pathway in the lungs of mice sub-chronically exposed to mainstream tobacco smokeReport as inadecuate




Induction of the interleukin 6- signal transducer and activator of transcription pathway in the lungs of mice sub-chronically exposed to mainstream tobacco smoke - Download this document for free, or read online. Document in PDF available to download.

BMC Medical Genomics

, 2:56

First Online: 21 August 2009Received: 31 October 2008Accepted: 21 August 2009

Abstract

BackgroundTobacco smoking is associated with lung cancer and other respiratory diseases. However, little is known about the global molecular changes that precede the appearance of clinically detectable symptoms. In this study, the effects of mainstream tobacco smoke MTS on global transcription in the mouse lung were investigated.

MethodsMale C57B1-CBA mice were exposed to MTS from two cigarettes daily, 5 days-week for 6 or 12 weeks. Mice were sacrificed immediately, or 6 weeks following the last cigarette. High density DNA microarrays were used to characterize global gene expression changes in whole lung. Microarray results were validated by Quantitative real-time RT-PCR. Further analysis of protein synthesis and function was carried out for a select set of genes by ELISA and Western blotting.

ResultsGlobally, seventy nine genes were significantly differentially expressed following the exposure to MTS. These genes were associated with a number of biological processes including xenobiotic metabolism, redox balance, oxidative stress and inflammation. There was no differential gene expression in mice exposed to smoke and sampled 6 weeks following the last cigarette. Moreover, cluster analysis demonstrated that these samples clustered alongside their respective controls. We observed simultaneous up-regulation of interleukin 6 IL-6 and its antagonist, suppressor of cytokine signalling SOCS3 mRNA following 12 weeks of MTS exposure. Analysis by ELISA and Western blotting revealed a concomitant increase in total IL-6 antigen levels and its downstream targets, including phosphorylated signal transducer and activator of transcription 3 Stat3, basal cell-lymphoma extra large BCL-XL and myeloid cell leukemia 1 MCL-1 protein, in total lung tissue extracts. However, in contrast to gene expression, a subtle decrease in total SOCS3 protein was observed after 12 weeks of MTS exposure.

ConclusionGlobal transcriptional analysis identified a set of genes responding to MTS exposure in mouse lung. These genes returned to basal levels following smoking cessation, providing evidence to support the benefits of smoking cessation. Detailed analyses were undertaken for IL-6 and its associated pathways. Our results provide further insight into the role of these pathways in lung injury and inflammation induced by MTS.

Electronic supplementary materialThe online version of this article doi:10.1186-1755-8794-2-56 contains supplementary material, which is available to authorized users.

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Author: Sabina Halappanavar - Marsha Russell - Martin R Stampfli - Andrew Williams - Carole L Yauk

Source: https://link.springer.com/







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