Otitis media: a genome-wide linkage scan with evidence of susceptibility loci within the 17q12 and 10q22.3 regionsReport as inadecuate




Otitis media: a genome-wide linkage scan with evidence of susceptibility loci within the 17q12 and 10q22.3 regions - Download this document for free, or read online. Document in PDF available to download.

BMC Medical Genetics

, 10:85

First Online: 03 September 2009Received: 16 June 2009Accepted: 03 September 2009

Abstract

BackgroundOtitis media OM is a common worldwide pediatric health care problem that is known to be influenced by genetics. The objective of our study was to use linkage analysis to map possible OM susceptibility genes.

MethodsUsing a stringent diagnostic model in which only those who underwent tympanostomy tube insertion at least once for recurrent-persistent OM are considered affected, we have carried out a genome-wide linkage scan using the 10K Affymetrix SNP panel. We genotyped 403 Caucasian families containing 1,431 genotyped individuals and 377 genotyped affected sib pairs, and 26 African American families containing 75 genotyped individuals and 27 genotyped affected sib pairs. After careful quality control, non-parametric linkage analysis was carried out using 8,802 SNPs.

ResultsIn the Caucasian-only data set, our most significant linkage peak is on chromosome 17q12 at rs226088 with a p-value of 0.00007. Other peaks of potential interest are on 10q22.3 0.00181 at rs1878001, 7q33 0.00105 at rs958408, 6p25.1 0.00261 at rs554653, and 4p15.2 0.00301 at rs2133507. In the combined Caucasian and African American dataset, the 10q22.3 peak becomes more significant, with a minimal p-value of 0.00026 at rs719871. Family-based association testing reveals signals near previously implicated genes: 513 kb from SFTPA2 10q22.3, 48 kb from IFNG 12q14, and 870 kb from TNF 6p21.3.

ConclusionOur scan does not provide evidence for linkage in the previously reported regions of 10q26.3 and 19q13.43. Our best-supported linkage regions may contain susceptibility genes that influence the risk for recurrent-persistent OM. Plausible candidates in 17q12 include AP2B1, CCL5, and a cluster of other CCL genes, and in 10q22.3, SFTPA2.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2350-10-85 contains supplementary material, which is available to authorized users.

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Author: Margaretha L Casselbrant - Ellen M Mandel - Jeesun Jung - Robert E Ferrell - Kathleen Tekely - Jin P Szatkiewicz - Amrit

Source: https://link.springer.com/



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