Dynamic changes in gene expression during human early embryo development: from fundamental aspects to clinical applications.Report as inadecuate




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* Corresponding author 1 Biothérapie des cellules souches normales et cancéreuses 2 Unité d-AMP-DPI, Département de Médecine et biologie de la reproduction 3 Unité de thérapie cellulaire

Abstract : BACKGROUND: The first week of human embryonic development comprises a series of events that change highly specialized germ cells into undifferentiated human embryonic stem cells hESCs that display an extraordinarily broad developmental potential. The understanding of these events is crucial to the improvement of the success rate of in vitro fertilization. With the emergence of new technologies such as Omics, the gene expression profiling of human oocytes, embryos and hESCs has been performed and generated a flood of data related to the molecular signature of early embryo development. METHODS: In order to understand the complex genetic network that controls the first week of embryo development, we performed a systematic review and study of this issue. We performed a literature search using PubMed and EMBASE to identify all relevant studies published as original articles in English up to March 2010 n = 165. We also analyzed the transcriptome of human oocytes, embryos and hESCs. RESULTS: Distinct sets of genes were revealed by comparing the expression profiles of oocytes, embryos on Day 3 and hESCs, which are associated with totipotency, pluripotency and reprogramming properties, respectively. Known components of two signaling pathways WNT and transforming growth factor-β were linked to oocyte maturation and early embryonic development. CONCLUSIONS: Omics analysis provides tools for understanding the molecular mechanisms and signaling pathways controlling early embryonic development. Furthermore, we discuss the clinical relevance of using a non-invasive molecular approach to embryo selection for the single-embryo transfer program.

Keywords : oocyte genes embryo genes embryo stem cells microarray non-invasive approach





Author: Said Assou - Imène Boumela - Delphine Haouzi - Tal Anahory - Hervé Dechaud - John De Vos - Samir Hamamah -

Source: https://hal.archives-ouvertes.fr/



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