Differences in transcriptomic profiles of human cumulus cells isolated from oocytes at GV, MI and MII stages after in vivo and in vitro oocyte maturation.Report as inadecuate




Differences in transcriptomic profiles of human cumulus cells isolated from oocytes at GV, MI and MII stages after in vivo and in vitro oocyte maturation. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Cellules souches normales et cancéreuses 2 Service de gynécologie-obstétrique, médecine de la reproduction Béclère 3 INSERM U1040 Cellules souches normales et cancéreuses, Service de biologie de la reproduction

Abstract : BACKGROUND Oocyte maturation and competence to development depends on its close relationship with cumulus cells CCs. However, the maturation conditions of human cumulus-oocyte complexes COCs might affect gene expression in both oocyte and CCs. We thus compared the transcriptome profiles of CCs isolated from in vivo and in vitro matured COCs at different nuclear maturation stages. METHODS Three groups of CCs from patients who underwent ICSI were included: CCs of patients with polycystic ovary syndrome PCOS referred for in vitro maturation IVM, CCs from patients with PCOS for in vivo maturation used as controls and CCs from normal responders referred for in vivo maturation. CCs were isolated from COCs at the germinal vesicle, metaphase I and metaphase II stages. Microarray technology was used to analyse the global gene expression and significance analysis of microarray to compare the expression profiles of CCs from COCs at different nuclear maturation stages following IVM or in vivo maturation. Selected genes were validated by RT-qPCR. RESULTS In CCs isolated after IVM, genes related to cumulus expansion and oocyte maturation, such as EREG, AREG and PTX3, were down-regulated, while cell cycle-related genes were up-regulated in comparison with CCs from in vivo matured COCs from PCOS and normal responder patients. Moreover, irrespective of the stage of oocyte maturation, genes involved in DNA replication, recombination and repair were up-regulated in CCs after IVM. CONCLUSIONS The CC transcriptomic signature varies according to both the oocyte maturation stage and the maturation conditions. Our findings suggest a delay in the acquisition of the mature CC phenotype following IVM, opening a new perspective for the improvement in IVM conditions.

Mots-clés : Cumulus cells DNA microarray gene expression profile maturation condition





Author: Zamalou Gisèle Ouandaogo - Nelly Frydman - Laetitia Hesters - Said Assou - Delphine Haouzi - Hervé Dechaud - René Frydman - Sa

Source: https://hal.archives-ouvertes.fr/



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