RNAi silencing of c-Myc inhibits cell migration, invasion, and proliferation in HepG2 human hepatocellular carcinoma cell line: c-Myc silencing in hepatocellular carcinoma cellReport as inadecuate




RNAi silencing of c-Myc inhibits cell migration, invasion, and proliferation in HepG2 human hepatocellular carcinoma cell line: c-Myc silencing in hepatocellular carcinoma cell - Download this document for free, or read online. Document in PDF available to download.

Cancer Cell International

, 13:23

First Online: 08 March 2013Received: 28 July 2012Accepted: 29 January 2013

Abstract

BackgroundHepatocellular carcinoma HCC is the most common type of liver cancer. Although much is known about both the cellular changes that lead to HCC and the etiological agents responsible for the majority of HCC cases, the molecule pathogenesis of HCC is still not well understood. We aimed to determine the effect of c-Myc gene expression on the proliferative, invasive, and migrative capabilities of hepatocellular carcinoma HepG2 cells.

MethodsA plasmid- based polymerase III promoter system was used to deliver and express short interfering RNA targeting c-Myc to reduce its expression in HepG2 cells. Western blot analysis was used to measure the protein level of c-Myc in HepG2 cells. The effects of c-Myc silencing on the invasion, motility, and proliferation of HepG2 cells were assessed using a Transwell chamber cell migration assay system and a growth curve assay, respectively.

ResultsThe data showed that plasmids expressing siRNA against c-Myc significantly decreased its expression in HepG2 cells by up to 85%. Importantly, pSilencer-c-Myc transfected cells showed a significantly reduced potential in migration, invasion, and proliferation.

ConclusionC-Myc plays an important role in the development of hepatocellular carcinoma. The data show that down-regulating the c-Myc protein level in HepG2 cells by RNAi could significantly inhibit migration, invasion and proliferation of HepG2 cells. Thus, c-Myc might be a potential therapeutic target for hepatocellular carcinoma.

KeywordsC-Myc Hepatic cancer HepG2 cells RNAi siRNA AbbreviationsHCCHepatocellular carcinoma

RNAiRNA interference

HATsHistone acetyltransferases

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-13-23 contains supplementary material, which is available to authorized users.

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Author: Yan Zhao - Wang Jian - Wei Gao - Ya-Xin Zheng - Yong-Kun Wang - Zhu-Qing Zhou - Hui Zhang - Cong-Jun Wang

Source: https://link.springer.com/







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