Germline TP53 mutational spectrum in French Canadians with breast cancerReport as inadecuate




Germline TP53 mutational spectrum in French Canadians with breast cancer - Download this document for free, or read online. Document in PDF available to download.

BMC Medical Genetics

, 16:24

Genetic epidemiology and genetic associations

Abstract

BackgroundSpecific germline mutations in the hereditary breast-ovarian cancer susceptibility HBC-HBOC genes, BRCA1, BRCA2 and PALB2, have been shown to recur in French Canadians of Quebec, Canada, and this has been attributed to common ancestors. Germline TP53 mutation carriers are known to segregate in Li-Fraumeni syndrome families, which feature young age of onset breast cancer. We have reported rare TP53 mutation carriers in French Canadian HBC families, though none recurred possibly due to the limited number of cancer families investigated. Here we describe TP53 germline mutations found in French Canadian cancer families provided from hereditary cancer clinics; investigate 37 new BRCA1 and BRCA2 mutation-negative HBC-HBOC families for the TP53 mutations; and assess the frequency of TP53 mutations in a 1235 French Canadian breast cancer cases not selected for family history of cancer.

MethodsTP53 mutation-positive pedigrees from French Canadian cancer families were provided from local hereditary cancer clinics. Bidirectional Sanger sequencing of all protein encoding exons of TP53 was performed using peripheral blood lymphocyte DNA from breast-ovarian cancer probands from 37 HBC-HBOC families of French Canadian descent. Targeted bidirectional Sanger sequencing assay of regions containing the identified TP53 mutations was performed on 1235 French Canadian breast cancer cases not selected for family history cancer.

ResultsFive new TP53 mutations were identified in six pedigrees from hereditary cancer clinics. No deleterious mutations were identified in cancer probands from 37 HBC-HBOC families. A targeted mutation screen of the 1235 breast cancer cases identified a c.844C>T p.Arg282Trp mutation carrier. This mutation was also found among the six mutation-positive cancer families provided by the local hereditary cancer clinics. The targeted screen also uncovered a new TP53 mutation, c.685T>C p.Cys229Arg that was found in two breast cancer cases. All TP53 mutation carriers were among the 656 women with breast cancer diagnosed less than 50 years of age.

ConclusionsIn all six new TP53 mutations were identified in French Canadians, where two each occurred in independently ascertained cases-families. Although all newly identified breast cancer mutation carriers reported a family history of cancer, none were consistent with features of Li-Fraumeni syndrome families.

KeywordsTP53 Breast cancer Hereditary breast cancer French Canadian Founders BRCA1 BRCA2  Download fulltext PDF



Author: Suzanna L Arcand - Mohammed R Akbari - Anne-Marie Mes-Masson - Diane Provencher - William D Foulkes - Steven A Narod - P

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents