Mifepristone improves chemo-radiation response in glioblastoma xenograftsReport as inadecuate




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Cancer Cell International

, 13:29

First Online: 25 March 2013Received: 23 December 2012Accepted: 18 March 2013

Abstract

BackgroundWe have investigated the ability of Mifepristone, an anti-progestin and anti-glucocorticoid drug, to modulate the antitumor effect of current standard clinical treatment in glioblastoma xenografts.

MethodsThe effect of radiation alone or combined with Mifepristone and Temozolamide was evaluated on tumor growth in glioblastoma xenografts, both in terms of preferentially triggering tumor cell death and inhibiting angiogenesis. Tumor size was measured once a week using a caliper and tumor metabolic-activity was carried out by molecular imaging using a microPET-CT scanner. The effect of Mifepristone on the expression of angiogenic factors after concomitant radio-chemotherapy was determined using a quantitative real-time PCR analysis of VEGF gene expression.

ResultsThe analysis of the data shows a significant antitumoral effect by the simultaneous administration of radiation-Mifepristone-Temozolamide in comparison with radiation alone or radiation-Temozolamide.

ConclusionOur results suggest that Mifepristone could improve the efficacy of chemo-radiotherapy in Glioblastoma. The addition of Mifepristone to standard radiation-Temozolamide therapy represents a potential approach as a chemo-radio-sensitizer in treating GBMs, which have very limited treatment options.

KeywordsGlioma Mifepristone Radiotherapy Chemo-radio-sensitizing AbbreviationsGBMGlioblastoma multiforme

DMEMDulbeccos modified Eagle’s medium

FCSFetal calf serum

EDTAEthylenediaminetetracetic acid

VOIVolume of Interest

RT-PCRReverse-transcriptase polymerase chain reaction

cdk2Cyclin-dependent kinase 2

BATBrown adipose tissue.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-13-29 contains supplementary material, which is available to authorized users.

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Author: Monserrat Llaguno-Munive - Luis Alberto Medina - Rafael Jurado - Mario Romero-Piña - Patricia Garcia-Lopez

Source: https://link.springer.com/



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