The Influence of Low Doses of Zearalenone on Distribution of Selected Active Substances in Nerve Fibers Within the Circular Muscle Layer of Porcine IleumReport as inadecuate




The Influence of Low Doses of Zearalenone on Distribution of Selected Active Substances in Nerve Fibers Within the Circular Muscle Layer of Porcine Ileum - Download this document for free, or read online. Document in PDF available to download.

Journal of Molecular Neuroscience

, Volume 56, Issue 4, pp 878–886

First Online: 15 March 2015Received: 03 November 2014Accepted: 27 February 2015

Abstract

The aim of this study was to investigate, whether low doses 25 % of no observable adverse effect levels values of zearalenone ZEN can affect the expression of active substances in nerve fibers in the muscular layer of porcine ileum. The study was performed on ten immature pigs divided into two groups: experimental group n = 5, where zearalenone 10 μg-kg body weight was given for 42 days, and control animals n = 5, where placebo was administered. Fragments of ileum of all animals were processed for single-labelling immunofluorescence technique using the antibodies against vasoactive intestinal peptide, neuronal form of nitric oxide synthase, cocaine and amphetamine regulatory peptide, galanin, pituitary adenylate cyclase-activating peptide-27 and substance P. The number of nerve fibers immunoreactive to particular substances was evaluated by the counting of nerves per observation field 0.1 mm. Low doses of zearalenone caused the clear changes in the expression of substances studied. The number of nerve fibers immunoreactive to the majority of substances increased in experimental animals. The exception was only galanin, the expression of which was less after administration of zearalenone. The obtained results for the first time show that even low doses of zearalenone can affect the nerve fibers in the digestive tract.

KeywordsZearalenone Mycotoxins Nerve fibers Gastrointestinal tract Immunohistochemistry Pig  Download fulltext PDF



Author: Sławomir Gonkowski - Kazimierz Obremski - Jaroslaw Calka

Source: https://link.springer.com/







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