CRISPR-Cas9 in zebrafish: an efficient combination for human genetic diseases modelingReport as inadecuate




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Human Genetics

, Volume 136, Issue 1, pp 1–12

First Online: 02 November 2016Received: 25 July 2016Accepted: 17 October 2016

Abstract

The next-generation sequencing identifies a growing number of candidate genes associated with human genetic diseases, which inevitably requires efficient methods to validate the causal links between genotype and phenotype. Recently, zebrafish, with sufficiently high-throughput capabilities, has become a favored option to study human pathogenesis. In addition, CRISPR-Cas9-based approaches have radically reduced the efforts to introduce targeted genome engineering in various organisms. Here, we systemically review the basic considerations in the design of gene editing in zebrafish with CRISPR-Cas9, and explore the potential of the combination of these two to support efficient functional analysis of human genetic variants.

J. Liu, Y. Zhou and X. Qi contributed equally to this study.

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Author: Jiaqi Liu - Yangzhong Zhou - Xiaolong Qi - Jia Chen - Weisheng Chen - Guixing Qiu - Zhihong Wu - Nan Wu

Source: https://link.springer.com/







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