Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta ratsReport as inadecuate




Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats - Download this document for free, or read online. Document in PDF available to download.

Genes and Environment

, 39:4

First Online: 06 January 2017Received: 07 September 2016Accepted: 08 December 2016

Abstract

IntroductionIt is known that fibrous particles of micrometer length, such as carbon nanotubes, which have same dimensions as asbestos, are carcinogenic. Carcinogenicity of nanomaterials is strongly related to inflammatory reactions; however, the genotoxicity mechanisms is unclear. Indeed, inconsistent results on genotoxicity of multi-walled carbon nanotubes MWCNTs have been shown in several reports. Therefore, we analyzed the in vivo genotoxicity induced by an intratracheal instillation of straight MWCNTs in rats using a different test system—the Pig-a gene mutation assay—that can reflect the genotoxicity occurring in the bone marrow. Since lungs were directly exposed to MWCNTs upon intratracheal instillation, we also performed the gpt assay using the lungs.

FindingsWe detected no significant differences in Pig-a mutant frequencies MFs between the MWCNT-treated and control rats. Additionally, we detected no significant differences in gpt MFs in the lung between the MWCNT-treated and control rats.

ConclusionsOur findings indicated that a single intratracheal instillation of MWCNTs was non-mutagenic to both the bone marrow and lung of rats.

KeywordsCarbon nanoparticle in vivo genotoxicity Pig-a gene mutation assay Multi-walled carbon nanotubes AbbreviationsC60Fullerene

CMCCarboxymethyl cellulose

ENUN-nitroso-N-ethylurea

FITCFluorescein isothiocyanate

GPIGlycosylphosphatidylinositol

HSCHematopoietic stem cell

MFMutant frequency

MWCNTsMulti-walled carbon nanotubes

RBCRed blood cell

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Author: Katsuyoshi Horibata - Akiko Ukai - Akio Ogata - Dai Nakae - Hiroshi Ando - Yoshikazu Kubo - Akemichi Nagasawa - Katsuhiro 

Source: https://link.springer.com/







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