A toxin antitoxin system promotes the maintenance of the IncA-C-mobilizable Salmonella Genomic Island 1Report as inadecuate




A toxin antitoxin system promotes the maintenance of the IncA-C-mobilizable Salmonella Genomic Island 1 - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 UR IASP - UR Infectiologie animale et Santé publique

Abstract : The multidrug resistance Salmonella Genomic Island 1 SGI1 is an integrative mobilizable element identified in several enterobacterial pathogens. This chromosomal island requires a conjugative IncA-C plasmid to be excised as a circular extrachromosomal form and conjugally mobilized in trans. Preliminary observations suggest stable maintenance of SGI1 in the host chromosome but paradoxically also incompatibility between SGI1 and IncA-C plasmids. Here, using a Salmonella enterica serovar Agona clonal bacterial population as model, we demonstrate that a Toxin-Antitoxin TA system encoded by SGI1 plays a critical role in its stable host maintenance when an IncA-C plasmid is concomitantly present. This system, designated sgiAT for Salmonella genomic island 1 Antitoxin and Toxin respectively, thus seems to play a stabilizing role in a situation where SGI1 is susceptible to be lost through plasmid IncA-C-mediated excision. Moreover and for the first time, the incompatibility between SGI1 and IncA-C plasmids was experimentally confirmed.





Author: Kévin T. Huguet - Mathieu Gonnet - Benoît Doublet - Axel Cloeckaert -

Source: https://hal.archives-ouvertes.fr/



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