ApoA-I-HDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in AstrocytesReport as inadecuate




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Journal of LipidsVolume 2014 2014, Article ID 530720, 9 pages

Review Article Biochemistry, Graduate School of Medical Sciences, Nagoya City University, Kawasumi 1, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan

Received 20 May 2014; Accepted 24 June 2014; Published 13 August 2014

Academic Editor: Akihiro Inazu

Copyright © 2014 Jinichi Ito and Makoto Michikawa. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Exogenous apolipoprotein A-I apoA-I associates with ATP-binding cassette transporter A1 ABCA1 on the cell surface of astrocytes like various peripheral cells and enhances the translocation of newly synthesized cholesterol from the endoplasmic reticulum-Golgi apparatus ER-Golgi to the cytosol. The cholesterol translocated to the cytosol is incorporated to cytosolic lipid-protein particles CLPP together with phospholipids and proteins such as sphingomyelin, phosphatidylcholine, caveolin-1, protein kinase Cα PK-Cα, and cyclophilin A. The CLPP are high density lipoproteins- HDL-like cytosolic lipid-protein complex with densities of 1.09–1.16 g-mL and diameters of 17-18 nm. The association of exogenous apoA-I with cellular ABCA1 induces tyrosine phosphorylation, activation, and translocation to the CLPP of ABCA1-associated phospholipase Cγ PL-Cγ in rat astrocytes. Furthermore, PK-Cα is translocated and activated to-in the CLPP through the production of diacylglyceride in the CLPP. ApoA-I enhances both the association of CLPP with microtubules and the phosphorylation of α-tubulin as a component of microtubules. The CLPP are dissociated from microtubules after α-tubulin in microtubules is phosphorylated by the CLPP-associated PK-Cα. The association and dissociation between CLPP and microtubules may participate in the intracellular transport of cholesterol to the plasma membrane.





Author: Jinichi Ito and Makoto Michikawa

Source: https://www.hindawi.com/



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