One SmpB molecule accompanies tmRNA during its passage through the ribosomes.Report as inadecuate




One SmpB molecule accompanies tmRNA during its passage through the ribosomes. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Belozersky Institute, Build. -A- 2 Department of Chemistry 3 Fonction, structure et inactivation d-ARN bactériens 4 Department of Genetics, Microbiology and Toxicology

Abstract : tmRNA and SmpB are the main participants of trans-translation, a process which rescues the ribosome blocked during translation of non-stop mRNA. While a one-to-one stoichiometry of tmRNA to the ribosome is generally accepted, the number of SmpB molecules in the complex is still under question. We have isolated tmRNA-ribosome complexes blocked at different steps of the tmRNA path through the ribosome and analyzed the stoichiometry of the complexes. Ribosome, tmRNA and SmpB were found in equimolar amount in the tmRNA-ribosome complexes stopped at the position of the 2nd, 4th, 5th or the 11th codons of the coding part of the tmRNA.

Keywords : Translation RNA-protein interaction Trans-translation tmRNA SmpB





Author: Elizaveta Bugaeva - Olga Shpanchenko - Brice Felden - Leif Isaksson - Olga Dontsova -

Source: https://hal.archives-ouvertes.fr/



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