Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targetsReport as inadecuate




Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targets - Download this document for free, or read online. Document in PDF available to download.

1 EMBL Heidelberg 2 IGDR - Institut de Génétique et Développement de Rennes 3 BSC - Biotechnologie et signalisation cellulaire

Abstract : Conventional techniques for single-base resolution mapping of epigenetic modifications of DNA such as 5-hydroxymethylcytosine 5hmC rely on the sequencing of bisulfite-modified DNA. Here we present an alternative approach called SCL-exo which combines selective chemical labeling SCL of 5hmC in genomic DNA with exonuclease exo digestion of the bead-trapped modified DNA molecules. Associated with a straightforward bioinformatic analysis, this new procedure provides an unbiased and fast method for mapping this epigenetic mark at high resolution. Implemented on mouse genomic DNA from in vitro-differentiated neural precursor cells, SCL-exo sheds light on an intrinsic lack of conservation of hydroxymethylated CpGs across vertebrates.

Keywords : 5hmC Single-CpG resolution Selective chemical labeling 5-hydroxymethylcytosine





Author: Aurélien A. Sérandour - Stéphane Avner - Elise A. Mahé - Thierry Madigou - Sylvain Guibert - Michaël Weber - Gilles Salbert

Source: https://hal.archives-ouvertes.fr/



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