Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin SupplementationReport as inadecuate

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BioMed Research InternationalVolume 2013 2013, Article ID 627907, 9 pages

Research ArticleInstituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apdo. Postal 70228, 04510 México, DF, Mexico

Received 14 April 2013; Accepted 2 July 2013

Academic Editor: Anastasia Kotanidou

Copyright © 2013 M. D. Ronquillo-Sánchez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Studies have found that biotin favors glucose and lipid metabolism, and medications containing biotin have been developed. Despite the use of biotin as a pharmacological agent, few studies have addressed toxicity aspects including the possible interaction with cytochrome P450 enzyme family. This study analyzed the effects of pharmacological doses of biotin on the expression and activity of the cytochrome P4501A subfamily involved in the metabolism of xenobiotics. Wistar rats were treated daily with biotin 2 mg-kg, i.p., while the control groups were treated with saline. All of the rats were sacrificed by cervical dislocation after 1, 3, 5, or 7 days of treatment. CYP1A1 and CYP1A2 mRNAs were modified by biotin while enzyme activity and protein concentration were not affected. The lack of an effect of biotin on CYP1A activity was confirmed using other experimental strategies, including i cotreatment of the animals with biotin and a known CYP1A inducer; ii the addition of biotin to the reaction mixtures for the measurement of CYP1A1 and CYP1A2 activities; and iii the use of an S9 mixture that was prepared from control and biotin-treated rats to analyze the activation of benzoapyrene BaP into mutagenic metabolites using the Ames test. The results suggest that biotin does not influence the CYP1A-mediated metabolism of xenobiotics.

Author: M. D. Ronquillo-Sánchez, R. Camacho-Carranza, C. Fernandez-Mejia, S. Hernández-Ojeda, M. Elinos-Baez, and J. J. Espinosa-Ag



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