Long-Term Ethanol Consumption Leadsto Lung Tissue Oxidative Stress and InjuryReport as inadecuate




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Oxidative Medicine and Cellular Longevity - Volume 3 2010, Issue 6, Pages 414-420



Department of Biochemistry, ES I-PGIMSR, Joka, Kolkata, India

Amrita Institute of Medical Sciences, Cochin, Kerala, India

Received 29 October 2010; Revised 25 November 2010; Accepted 6 December 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Alcohol abuse is a systemic disorder. The deleterious health effects of alcohol consumption may result in irreversible organ damage. By contrast, there currently is little evidence for the toxicity of chronic alcohol use on lung tissue. Hence, in this study we investigated long-term effects of ethanol in the lung.

Results: Though body weight of rats increased significantly with duration of exposure compared to its initial weight, there was no significant change in relative weight g-100 g body weight of lung due to ethanol exposure. The levels of thiobarbituric acid reactive substances TBARS, nitrite, protein carbonyl, oxidized glutathione GSS G, redox ratio GSS G- GSH and GST activity elevated; while reduced glutathione GSH level and activities of glutathione reductase GR, glutathione peroxidase GPx, catalase, superoxide dismutase SOD and Na

K

ATPase reduced significantly with duration of ethanol exposure in the lung homogenate compared to the control group. Total matrix metalloproteinase activity elevated in the lung homogenate with time of ethanol consumption. Histopathologic examination also demonstrated that severity of lung injury enhanced with duration of ethanol exposure.

Methods: 16–18 week-old male albino Wistar strain rats weighing 200–220 g were fed with ethanol 1.6 g-kg body weight-day up to 36 weeks. At the end of the experimental period, blood samples were collected from reteroorbital plexus to determine blood alcohol concentration and the animals were sacrificed. Various oxidative stress-related biochemical parameters, total matrix metalloproteinase activity and histopathologic examinations of the lung tissues were performed.

Conclusions: Results of this study indicate that long-term ethanol administration aggravates systemic and local oxidative stress, which may be associated with lung tissue injury.





Author: Subir Kumar Das and Sukhes Mukherjee

Source: https://www.hindawi.com/



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