Upregulation of EMMPRIN OX47 in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve InjuryReport as inadecuate




Upregulation of EMMPRIN OX47 in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury - Download this document for free, or read online. Document in PDF available to download.

Neural PlasticityVolume 2015 2015, Article ID 249756, 9 pages

Research Article

Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

Department of Anesthesiology, Yulin First Hospital, Yulin 710021, China

Institute of Basic Medical Science, Xi’an Medical University, Xi’an 710021, China

Experiment Center of Biomedical Research, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China

Received 28 April 2015; Revised 24 June 2015; Accepted 28 June 2015

Academic Editor: Yun Guan

Copyright © 2015 Qun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Matrix metalloproteinases MMPs are widely implicated in inflammation and tissue remodeling associated with various neurodegenerative diseases and play an important role in nociception and allodynia. Extracellular Matrix Metalloproteinase Inducer EMMPRIN plays a key regulatory role for MMP activities. However, the role of EMMPRIN in the development of neuropathic pain is not clear. Western blotting, real-time quantitative RT-PCR qRT-PCR, and immunofluorescence were performed to determine the changes of messenger RNA and protein of EMMPRIN-OX47 and their cellular localization in the rat dorsal root ganglion DRG after nerve injury. Paw withdrawal threshold test was examined to evaluate the pain behavior in spinal nerve ligation SNL model. The lentivirus containing OX47 shRNA was injected into the DRG one day before SNL. The expression level of both mRNA and protein of OX47 was markedly upregulated in ipsilateral DRG after SNL. OX47 was mainly expressed in the extracellular matrix of DRG. Administration of shRNA targeted against OX47 in vivo remarkably attenuated mechanical allodynia induced by SNL. In conclusion, peripheral nerve injury induced upregulation of OX47 in the extracellular matrix of DRG. RNA interference against OX47 significantly suppressed the expression of OX47 mRNA and the development of mechanical allodynia. The altered expression of OX47 may contribute to the development of neuropathic pain after nerve injury.





Author: Qun Wang, Yanyuan Sun, Yingna Ren, Yandong Gao, Li Tian, Yang Liu, Yanan Pu, Xingchun Gou, Yanke Chen, and Yan Lu

Source: https://www.hindawi.com/



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