The Binding of EGFR to GM13 Hosted in Lipid Raft-Like Biomembranes Insighted by Plasmonic Resonance TechniquesReport as inadecuate




The Binding of EGFR to GM13 Hosted in Lipid Raft-Like Biomembranes Insighted by Plasmonic Resonance Techniques - Download this document for free, or read online. Document in PDF available to download.

Journal of Sensors - Volume 2015 2015, Article ID 282458, 8 pages -

Research Article

Institute for Complex Systems of the National Council of Researches, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy

Department of Experimental and Clinical Biomedical Sciences, Section of Experimental Pathology and Oncology, University of Florence, Viale G.B.
Morgagni 50, 50134 Florence, Italy

Departamento de Física, Pontificia Universidade Catolica Rio Prédio Cardeal Leme, Sala 652, Rua Marquês de São Vicente 225, 22451-900 Gávea, RJ, Brazil

Received 4 June 2015; Accepted 14 July 2015

Academic Editor: Banshi D. Gupta

Copyright © 2015 Giancarlo Margheri et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We exploit Au-SiO2 plasmonic structures to check the effective binding activity of GM13 gangliosides hosted in physiological-like biomembranes, in presence of the Epidermal Growth Factor Receptor EGFR.
To this aim, we used bilayers that support the propagation of optical surface plasmon modes plasmonic transducers, PTs or guided modes Plasmon Waveguide Resonators, PWRs.
First, we measured the binding of EGFR to GM13 by using PTs.
Indeed, effective interactions were evidenced, but with faint signals that prevented resolving dissociation kinetics.
In order to enhance the optical responses, we turned our attention to PWRs.
We first refined the design of a previously adopted Au-SiO2 PWR, finding that the nominal sensitivity is independent on SiO2 thickness but strongly dependent on its residual losses, due typically to a nonoptimal deposition process.
We fabricated an improved Au-SiO2 resonator and tested the predicted signal enhancement by monitoring the binding of EGFR to GM3-enriched biomembranes.
The measured signal was ~12-fold higher than that one measured using a PT, close to the maximum theoretical enhancement.
The higher PWR response enabled us to detect the dissociation of EGFR from GM3, and the value of the apparent dissociation constant of the GM3-EGFR complex could be obtained.





Author: Giancarlo Margheri, Silvana Trigari, Stefano Sottini, Riccardo D’Agostino, Tommaso Del Rosso, and Mario Del Rosso

Source: https://www.hindawi.com/



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