Aged Tgfβ2-Gdnf double-heterozygous mice show no morphological and functional alterations in the nigrostriatal systemReport as inadecuate




Aged Tgfβ2-Gdnf double-heterozygous mice show no morphological and functional alterations in the nigrostriatal system - Download this document for free, or read online. Document in PDF available to download.

Journal of Neural Transmission

, Volume 117, Issue 6, pp 719–727

First Online: 11 May 2010Received: 05 October 2009Accepted: 18 April 2010

Abstract

Loss of dopaminergic neurons in the substantia nigra pars compacta and the resulting decrease in striatal dopamine levels are the hallmarks of Parkinson’s disease. Tgfβ and Gdnf have been identified as neurotrophic factors for dopaminergic midbrain neurons in vivo and in vitro. Haploinsufficiency for either Tgfβ or Gdnf led to dopaminergic deficits. In this study we therefore analyzed the nigrostriatal system of aged Tgfβ2-Gdnf double-heterozygous mice. Unexpectedly, we found no morphological changes in the nigrostriatal system as compared with age-matched wild-type mice. There were no significant differences in the number of TH-positive midbrain neurons and no changes in the optical density of TH immunoreactivity in striata of Tgfβ2-Gdnf double-heterozygous mice. Moreover, we found no significant differences in the striatal levels of dopamine and its metabolites dihydroxyphenylacetic acid and homovanillic acid. Our results indicate that a combined haploinsufficiency for Tgfβ2 and Gdnf has no impact on the function and the survival of midbrain DA neurons under normal aging conditions.

KeywordsTgfβ2 Gdnf Dopamine Nigro-striatal system Midbrain  Download fulltext PDF



Author: Stephan Heermann - Felipe Opazo - Björn Falkenburger - Kerstin Krieglstein - Björn Spittau

Source: https://link.springer.com/







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