An Automated 96-Well-Plate Loader for the FACScanⓇReport as inadecuate




An Automated 96-Well-Plate Loader for the FACScanⓇ - Download this document for free, or read online. Document in PDF available to download.

Analytical Cellular Pathology - Volume 19 1999, Issue 2, Pages 99-103

Technical Note University of Konstanz, Faculty of Biology, Department of Immunology, Konstanz, Germany

Received 24 June 1999; Accepted 12 October 1999

Copyright © 1999 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Staining multiple samples for data acquisition with a flow cytometer is done in 96‐well plates to save time and make large data assessment in one working day possible. However, the inability of the FACScan

to take up the samples from 96‐well plates is a major drawback. In order to avoid the individual transfer of samples to tubes, we have developed a system, which allows using the FACScan

with 96‐well plates. The machine consists of a programmable control module and a loader which moves the 96‐well plate in 3 axis well by well along the sample collector. The machine is equipped with a wash buffer tank to avoid cross contamination of samples and a shaking option to avoid sedimentation of cells during acquisition. The machine can be further developed into a full automatic loader if connected to the FACS Station. In 24 hours about 7,000 samples with 10,000 cells each can be acquired.





Author: Harald Illges

Source: https://www.hindawi.com/



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