Mesenchymal Stromal Cell-Derived Factors Promote Tissue Repair in a Small-for-Size Ischemic Liver Model but Do Not Protect against Early Effects of Ischemia and Reperfusion InjuryReport as inadecuate




Mesenchymal Stromal Cell-Derived Factors Promote Tissue Repair in a Small-for-Size Ischemic Liver Model but Do Not Protect against Early Effects of Ischemia and Reperfusion Injury - Download this document for free, or read online. Document in PDF available to download.

Journal of Immunology Research - Volume 2015 2015, Article ID 202975, 13 pages -

Research Article

Department of Surgery, Erasmus MC-University Medical Center, 3015 CE Rotterdam, Netherlands

Department of Surgery, Penn Transplant Institute, University of Pennsylvania, Philadelphia, PA 19104, USA

Department of Transplant Immunology, Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104-4318, USA

Received 9 April 2015; Accepted 27 May 2015

Academic Editor: Bjarne K. Møller

Copyright © 2015 Suomi M. G. Fouraschen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Loss of liver mass and ischemia-reperfusion injury IRI are major contributors to postresectional liver failure and small-for-size syndrome. Mesenchymal stromal cell- MSC- secreted factors are described to stimulate regeneration after partial hepatectomy. This study investigates if liver-derived MSC-secreted factors also promote liver regeneration after resection in the presence of IRI. C57BL-6 mice underwent IRI of 70% of their liver mass, alone or combined with 50% partial hepatectomy PH. Mice were treated with MSC-conditioned medium MSC-CM or unconditioned medium UM and sacrificed after 6 or 24 hours IRI group or after 48 hours IRI + PH group. Blood and liver tissue were analyzed for tissue injury, hepatocyte proliferation, and gene expression. In the IRI alone model, serum ALT and AST levels, hepatic tissue damage, and inflammatory cytokine gene expression showed no significant differences between both treatment groups. In the IRI + PH model, significant reduction in hepatic tissue damage as well as a significant increase in hepatocyte proliferation was observed after MSC-CM treatment. Conclusion. Mesenchymal stromal cell-derived factors promote tissue regeneration of small-for-size livers exposed to ischemic conditions but do not protect against early ischemia and reperfusion injury itself. MSC-derived factors therefore represent a promising treatment strategy for small-for-size syndrome and postresectional liver failure.





Author: Suomi M. G. Fouraschen, Joshua H. Wolf, Luc J. W. van der Laan, Petra E. de Ruiter, Wayne W. Hancock, Job P. van Kooten, Mon

Source: https://www.hindawi.com/



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