The diagnostic value of matrix metalloproteinase 9 MMP-9 and tissue inhibitor of matrix metalloproteinases 1 TIMP-1 determination in the sera of colorectal adenoma and cancer patientsReport as inadecuate




The diagnostic value of matrix metalloproteinase 9 MMP-9 and tissue inhibitor of matrix metalloproteinases 1 TIMP-1 determination in the sera of colorectal adenoma and cancer patients - Download this document for free, or read online. Document in PDF available to download.

International Journal of Colorectal Disease

, Volume 25, Issue 10, pp 1177–1184

First Online: 17 June 2010Accepted: 04 June 2010

Abstract

PurposeTumor cells, including colorectal cancer CRC, are able to produce and release matrix metalloproteinase 9 MMP-9 which is involved in tumor invasion and metastasis. Natural tissue inhibitors of matrix metalloproteinases TIMPs regulate activity of MMPs and stimulate tumor growth and malignant transformation. The aim of the present study was to compare the clinical significance of serum MMP-9 with TIMP-1 in the diagnosis of CRC patients and in the differentiation between colorectal adenoma CA and cancer.

MethodsSerum MMP-9 and TIMP-1 were measured in 75 CRC patients, 35 CA, and 70 healthy subjects using enzyme-linked immunosorbent assay. Concentrations of carcinoembryonic antigen CEA and carbohydrate antigen 19-9 CA 19-9 were determined by microparticle enzyme immunoassay.

ResultsSerum levels of all proteins tested were significantly higher in CRC patients than in healthy subjects. Additionally, serum TIMP-1 was significantly higher in patients with CRC than in CA patients. Concentrations of TIMP-1 correlated with tumor stage, nodal involvement, presence of distant metastases, patients- survival, and tumor resectability. Diagnostic sensitivity of TIMP-1 was higher 61% than those of other biomarkers MMP-9, 55%; CEA, 39%; CA 19-9, 11%, and increased in combined use with MMP-9 75% or CEA 73%. The areas under receiver operating characteristic curves of TIMP-1 were larger than those of MMP-9.

ConclusionsOur findings suggest better usefulness of serum TIMP-1 than MMP-9 in the diagnosis of CRC, especially in the assessment of Duke-s classification of tumor stage, survival of cancer patients, resectability of tumor, and in the differentiation between CA and cancer.

KeywordsColorectal cancer Colorectal adenoma MMP-9 TIMP-1 Tumor markers  Download fulltext PDF



Author: Barbara Mroczko - Magdalena Groblewska - Bogna Okulczyk - Bogusław Kędra - Maciej Szmitkowski

Source: https://link.springer.com/article/10.1007/s00384-010-0991-9







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