Replication of linkage at chromosome 20p13 and identification of suggestive sex-differential risk loci for autism spectrum disorderReport as inadecuate




Replication of linkage at chromosome 20p13 and identification of suggestive sex-differential risk loci for autism spectrum disorder - Download this document for free, or read online. Document in PDF available to download.

Molecular Autism

, 5:13

First Online: 17 February 2014Received: 27 August 2013Accepted: 24 January 2014

Abstract

BackgroundAutism spectrum disorders ASDs are male-biased and genetically heterogeneous. While sequencing of sporadic cases has identified de novo risk variants, the heritable genetic contribution and mechanisms driving the male bias are less understood. Here, we aimed to identify familial and sex-differential risk loci in the largest available, uniformly ascertained, densely genotyped sample of multiplex ASD families from the Autism Genetics Resource Exchange AGRE, and to compare results with earlier findings from AGRE.

MethodsFrom a total sample of 1,008 multiplex families, we performed genome-wide, non-parametric linkage analysis in a discovery sample of 847 families, and separately on subsets of families with only male, affected children male-only, MO or with at least one female, affected child female-containing, FC. Loci showing evidence for suggestive linkage logarithm of odds ≥2.2 in this discovery sample, or in previous AGRE samples, were re-evaluated in an extension study utilizing all 1,008 available families. For regions with genome-wide significant linkage signal in the discovery stage, those families not included in the corresponding discovery sample were then evaluated for independent replication of linkage. Association testing of common single nucleotide polymorphisms SNPs was also performed within suggestive linkage regions.

ResultsWe observed an independent replication of previously observed linkage at chromosome 20p13 P < 0.01, while loci at 6q27 and 8q13.2 showed suggestive linkage in our extended sample. Suggestive sex-differential linkage was observed at 1p31.3 MO, 8p21.2 FC, and 8p12 FC in our discovery sample, and the MO signal at 1p31.3 was supported in our expanded sample. No sex-differential signals met replication criteria, and no common SNPs were significantly associated with ASD within any identified linkage regions.

ConclusionsWith few exceptions, analyses of subsets of families from the AGRE cohort identify different risk loci, consistent with extreme locus heterogeneity in ASD. Large samples appear to yield more consistent results, and sex-stratified analyses facilitate the identification of sex-differential risk loci, suggesting that linkage analyses in large cohorts are useful for identifying heritable risk loci. Additional work, such as targeted re-sequencing, is needed to identify the specific variants within these loci that are responsible for increasing ASD risk.

KeywordsMale brain Sex differences Intermediate phenotype Linkage analysis Association AGRE AbbreviationsAGREAutism Genetics Resource Exchange

ALLall families

ASDautism spectrum disorders

CNVcopy number variant

FCfemale-containing

ILinterleukin

LODlogarithm of odds

MOmale-only

SNPsingle nucleotide polymorphism

SNVsingle nucleotide variant

TDTtransmission disequilibrium test.

Electronic supplementary materialThe online version of this article doi:10.1186-2040-2392-5-13 contains supplementary material, which is available to authorized users.

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Author: Donna M Werling - Jennifer K Lowe - Rui Luo - Rita M Cantor - Daniel H Geschwind

Source: https://link.springer.com/







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