Polimixina b: efeito dose e tempo dependente na nefrotoxicidade in vitro Report as inadecuate




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Cassiane Dezoti Fonseca ; Mirian Watanabe ; Maria de Fátima Fernandes Vattimo ;Acta Paulista de Enfermagem 2013, 26 1

Author: Luciana Barros de Moura Neiva

Source: http://www.redalyc.org/articulo.oa?id=307026771011_2


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Red de Revistas Científicas de América Latina, el Caribe, España y Portugal Sistema de Información Científica English version Neiva, Luciana Barros de Moura; Fonseca, Cassiane Dezoti; Watanabe, Mirian; Vattimo, Maria de Fátima Fernandes Polimixina B: efeito dose e tempo dependente na nefrotoxicidade in vitro Acta Paulista de Enfermagem, vol.
26, núm.
1, enero-febrero, 2013, pp.
57-62 Escola Paulista de Enfermagem São Paulo, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=307026771011 Acta Paulista de Enfermagem, ISSN (Printed Version): 0103-2100 lvianna@unifesp.br Escola Paulista de Enfermagem Brasil How to cite Complete issue More information about this article Journals homepage www.redalyc.org Non-Profit Academic Project, developed under the Open Acces Initiative Original Article Polymyxin B: dose and time dependent nephrotoxicity effect in vitro Polimixina B: efeito dose e tempo dependente na nefrotoxicidade in vitro Luciana Barros de Moura Neiva1 Cassiane Dezoti da Fonseca1 Mirian Watanabe1 Maria de Fátima Fernandes Vattimo1 Keywords Polymyxin B; Acute kidney injury; LLCPK1 cells; Apoptosis Descritores Polimixina B; Lesão renal aguda; Células LLC-PK1; Apoptose Submitted April 19, 2012 Accepted February 21, 2013 Abstract Objective: To characterize the toxicity of polymyxin B (PmxB) in renal cell in different dosage and times. Methods: LLC-PK1 cells grown in 12 well multiwell plates were divided into the following groups: Control (CTL) - cells maintained in DMEM supplemented with 5%; G1 - cells exposed to concentration of 75µM PmxB G2 - cells exposed to concentration of 375µM PmxB.
Each group was assessed at 24,48 and 72 hours as for cell viability (Acridine orange-ethidium bromide) and apoptosis (Hoechst 33342). Results: The data demonstrate the cell viability and apoptosis exposure of three doses of PmxB in three time intervals, with a significant increase in toxicity to high doses and longer duration of stay in the antibi...





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