Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging.Report as inadecuate




Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 ICSN - Institut de Chimie des Substances Naturelles 2 NBCM - Laboratoire de neurobiologie cellulaire et moléculaire 3 INAF - Institut de Neurobiologie Alfred Fessard

Abstract : Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry cluster-ToF-SIMS imaging using a 25 keV Bi3+ liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 x 500 microm2 image resolution, 256 x 256 pixels. Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.

Keywords : Duchenne muscular dystrophy human skeletal muscle degeneration lipidomics time-of-flight secondary ion mass spectrometry oxidative stress regeneration





Author: Nora Tahallah - Alain Brunelle - Sabine De La Porte - Olivier Laprévote -

Source: https://hal.archives-ouvertes.fr/



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