Impact of Y chromosome AZFc subdeletion shows lower risk of fertility impairment in Siddi tribal men, Western Ghats, IndiaReport as inadecuate




Impact of Y chromosome AZFc subdeletion shows lower risk of fertility impairment in Siddi tribal men, Western Ghats, India - Download this document for free, or read online. Document in PDF available to download.

Basic and Clinical Andrology

, 25:1

First Online: 22 January 2015Received: 08 October 2014Accepted: 15 December 2014

Abstract

BackgroundIndia is characterized by the presence of a large number of endogamous castes, tribes and religions, having second largest concentration of tribal population in the World with differed genetic ethnicity, lifestyle and environmental habitat from those of mainstream population. Lack of data is constraint when it comes to tracking the tribal population health status, specifically reproductive health aspects by experimental approaches. The male fertility impairment depends on Y chromosome azoospermia factor c AZFc subdeletions, which varies highly in different geographical populations and in an Indian admixed population the frequency and effect of deletion on fertility is relatively poorly documented. Therefore, the current study has been initiated to enumerate and characterize the strength of association between Yq11 AZFc subdeletions and fertility impairment among Siddi tribal men of Western Ghats, India.

MethodsHere, using predesigned performa we collected personal as well as familial information of 200 volunteered male subjects and grouped them into: i 104 married individuals with proven fertility, and ii 96 unmarried men with unknown fertility status. Quantification of reproductive hormones such as follicle stimulating hormone FSH, leutinizing hormone LH and testosterone were studied. Oxidative stress markers like total antioxidant capacity TAC and super oxide dismutase SOD along with analysis of five sequence tagged site STS hotspot markers were employed for mapping of Y chromosome AZFc subdeletions. Statistical analyses were performed using SPSS software.

ResultsHormonal analysis and estimation of oxidative stress markers showed normal values with no significant differences between two subgroups. However, the Y chromosome AZFc subdeletion mapping revealed evident results as an individual displayed absence of STS sY1191 marker indicating b2-b3 deletion, whereas rest of the subjects exhibited no deletion for all the five STS markers. While, the individual has fathered two children, at this point it is difficult to draw a causal link between the observed deletion and its effect on fertility.

ConclusionThus, our current study suggests that the association between AZFc subdeletions with its effect on infertility varies highly in this study cohort compared to other Indian ethnic groups, exhibiting lower risk factor and non-association reaching insignificance among Siddi tribal men.

KeywordsMale infertility Y chromosome Subdeletions Siddi tribe Ethnicity AbbreviationsAZFcAzoospermic factor c

AZFAzoospermic factor

FSHFollicle stimulating hormone

LHLeutinizing hormone

TACTotal antioxidant capacity

SODSuper oxide dismutase

STSSequence tagged site

MSYMale-specifc Y

TCATrichloroaceticacid

BPY2Basic protein Y

CDYChromodomain Y

DAZDeletion in azoospermia

GOLGAZLYGolgi autoantigen golgin subfamily a2 like Y

CSPG4LYChondroitin sulphate proteoglycan 4 like Y

TTYTestis transcript Y

EAAEuropean academy of andrology

EQMNEuropean molecular genetics quality network

ROSReactive oxygen species

IHECInstitutional human ethical committee

ODOptical density

STDSexually transmitted disease

Electronic supplementary materialThe online version of this article doi:10.1186-s12610-014-0017-5 contains supplementary material, which is available to authorized users.

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Author: Shivaprasad Holenarasipura Sathyanarayana - Suttur Srikanta Naik Malini

Source: https://link.springer.com/article/10.1186/s12610-014-0017-5



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