Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African PopulationReport as inadecuate




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Mediators of Inflammation - Volume 2014 2014, Article ID 217019, 9 pages -

Research Article

Division of Chemical Pathology, Faculty of Health Sciences, National Health Laboratory Service NHLS and University of Stellenbosch, Cape Town 7505, South Africa

Non-Communicable Diseases Research Unit, South African Medical Research Council, University of Cape Town, Cape Town 7505, South Africa

Division of Chemical Pathology, University of Cape Town, Cape Town 8000, South Africa

Department of Biomedical Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa

Received 9 July 2014; Revised 20 October 2014; Accepted 21 October 2014; Published 16 November 2014

Academic Editor: Vinod K. Mishra

Copyright © 2014 M. Macharia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Paraoxonase 1 PON1 activity is markedly influenced by coding polymorphisms, Q-R at position 192 and M-L at position 55 of the PON1 gene. We investigated the frequencies of these polymorphisms and their effects on PON1 and antioxidant activities in 844 South African mixed ancestry individuals. Genotyping was done using allele-specific TaqMan technology, PON1 activities were measured using paraoxon and phenylacetate, oxidative status was determined by measuring the antioxidant activities of ferric reducing antioxidant power and trolox equivalent antioxidant capacity, and lipid peroxidation markers included malondialdehyde and oxidized LDL. The frequencies of Q192R and L55M were 47.6% and 28.8%, respectively, and the most common corresponding alleles were 192R 60.4% and 55M 82.6%. The Q192 was significantly associated with 5.8 units’ increase in PON1 concentration and 15.4 units’ decrease in PONase activity after adjustment for age, sex, BMI, and diabetes, with suggestion of differential effects by diabetes status. The PON1 L55 variant was associated with none of the measured indices. In conclusion, we have shown that the Q192R polymorphism is a determinant of both PON1 concentration and activity and this association appeared to be enhanced in subjects with diabetes.





Author: M. Macharia, A. P. Kengne, D. M. Blackhurst, R. T. Erasmus, and T. E. Matsha

Source: https://www.hindawi.com/



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