Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean SeaReport as inadecuate




Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 LOBB - Laboratoire d-océanographie biologique de Banyuls 2 DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Abstract : A novel aerobic, heterotrophic, prosthecate bacterium, designated 26III-A02-215T, was isolated from surface water of the north-western Mediterranean Sea. Cells stained Gram-negative and were straight to slightly curved rods, forming red colonies on agar plates. The strain grew at 15-37 degrees C inclusive optimum 30 degrees C and grew optimally at seawater salinity. Growth was observed on organic acids, amino acids and complex organic substrates. The fatty acids >5 % detected in strain 26III-A02-215T were C17 : 1omega6c, C18 : 1omega7c and C17 : 0. The lipid pattern indicated the presence of phosphatidylglycerol, glucuronopyranosyldiglyceride, monoglycosyldiglyceride, an unidentified glycolipid and three unidentified phospholipids. Phosphatidylethanolamine and diphosphatidylglycerol were absent. Ubiquinone Q-10 was the only respiratory lipoquinone. The G+C content of the genomic DNA was 46.8 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain 26III-A02-215T belonged to the Hyphomonas-Hirschia-Robiginitomaculum branch of the order Caulobacterales. This affiliation was consistent with the results of polar lipid analyses. Among this group, the novel isolate was most closely related to Robiginitomaculum antarcticum 93.9 % 16S rRNA gene sequence similarity to the type strain. On the basis of genotypic, chemotaxonomic and phenotypic distinctness, we propose a novel genus, Hellea gen. nov., with Hellea balneolensis sp. nov. as the type species. The type strain of Hellea balneolensis is 26III-A02-215T =DSM 19091T =CIP 109500T =OOB 269T.

Keywords : Marine environment Bacteria Mediterranean Sea Taxonomy New species New genus





Author: Karine Alain - Brian J. Tindall - Laurent Intertaglia - Philippe Catala - Philippe Lebaron -

Source: https://hal.archives-ouvertes.fr/



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