Preparation and Characterization of Novel PBAE-PLGA Polymer Blend Microparticles for DNA Vaccine DeliveryReport as inadecuate




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The Scientific World Journal - Volume 2014 2014, Article ID 385135, 9 pages -

Research Article

Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India

College of Pharmacy-Boys, Shaqra University, Al Dawadmi Campus, Al Dawadmi 11911, Saudi Arabia

Directorate of Research, Manipal University, Manipal 576104, India

Received 11 July 2014; Revised 16 September 2014; Accepted 18 September 2014; Published 27 October 2014

Academic Editor: Xiao-Qi Yu

Copyright © 2014 Meenashi Vanathi Balashanmugam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Context. Polybeta-amino ester PBAE with its pH sensitiveness and Polylactic-co-glycolic acid PLGA with huge DNA cargo capacity in combination prove to be highly efficient as DNA delivery system. Objective. To study the effectiveness of novel synthesized PBAE polymer with PLGA blend at different ratios in DNA vaccine delivery. Methods. In the present study, multifunctional polymer blend microparticles using a combination of PLGA and novel PBAE polymers A1 bis3-propionyloxypropyl3,3′-propane-1,3-diyl-bismethylazanediyldipropanoate and A2 bis4-propionyloxybutyl3,3′-ethane-1,2-diyl-bisisopropylazanediyldipropanoate at different ratios 85 : 15, 75 : 25, and 50 : 50 were prepared by double emulsion solvent removal method. The microparticles were characterized for cytotoxicity, transfection efficiency, and DNA encapsulation efficiency. Result. It was evident from results that among the microparticles prepared with PLGA-PBAE blend the PLGA : PBAE at 85 : 15 ratio was found to be more effective combination than the microparticles prepared with PLGA alone in terms of transfection efficiency and better DNA integrity. Microparticles made of PLGA and PBAE A1 at 85 : 15 ratio, respectively, were found to be less toxic when compared with microparticles prepared with A2 polymer. Conclusion. The results encourage the use of the synthesized PBAE polymer in combination with PLGA as an effective gene delivery system.





Author: Meenashi Vanathi Balashanmugam, Sivagurunathan Nagarethinam, Hitesh Jagani, Venkata Rao Josyula, Abdulmohsen Alrohaimi, and N

Source: https://www.hindawi.com/



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