The Anti-Inflammatory Effect of Human Telomerase-Derived Peptide on P. gingivalis Lipopolysaccharide-Induced Inflammatory Cytokine Production and Its Mechanism in Human Dental Pulp CellsReport as inadecuate




The Anti-Inflammatory Effect of Human Telomerase-Derived Peptide on P. gingivalis Lipopolysaccharide-Induced Inflammatory Cytokine Production and Its Mechanism in Human Dental Pulp Cells - Download this document for free, or read online. Document in PDF available to download.

Mediators of Inflammation - Volume 2015 2015, Article ID 385127, 8 pages -

Research Article

Department of Conservative Dentistry, Dental Research Institute and School of Dentistry, Seoul National University, Jongno-gu, Yeongun-dong 275-1, Seoul 110-749, Republic of Korea

Department of Family Medicine, Eulji University School of Medicine, Joong-gu, Yongdu-dong 143-5, Daejeon, Republic of Korea

School of Dentistry, David Geffen School of Medicine, Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA 90095, USA

Received 24 June 2015; Revised 9 October 2015; Accepted 13 October 2015

Academic Editor: Jean-Christophe Farges

Copyright © 2015 Yoo-Jin Ko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Porphyromonas gingivalis is considered with inducing pulpal inflammation and has lipopolysaccharide LPS as an inflammatory stimulator. GV1001 peptide has anticancer and anti-inflammation activity due to inhibiting activation of signaling molecules after penetration into the various types of cells. Therefore, this study examined inhibitory effect of GV1001 on dental pulp cells hDPCs stimulated by P. gingivalis LPS. The intracellular distribution of GV1001 was analyzed by confocal microscopy. Real-time RT-PCR was performed to determine the expression levels of TNF-α and IL-6 cytokines. The role of signaling by MAP kinases ERK and p38 was explored using Western blot analysis. The effect of GV1001 peptide on hDPCs viability was measured by MTT assay. GV1001 was predominantly located in hDPC cytoplasm. The peptide inhibited P. gingivalis LPS-induced TNF-α and IL-6 production in hDPCs without significant cytotoxicity. Furthermore, GV1001 treatment markedly inhibited the phosphorylation of MAP kinases ERK and p38 in LPS-stimulated hDPCs. GV1001 may prevent P. gingivalis LPS-induced inflammation of apical tissue. Also, these findings provide mechanistic insight into how GV1001 peptide causes anti-inflammatory actions in LPS-stimulated pulpitis without significantly affecting cell viability.





Author: Yoo-Jin Ko, Kil-Young Kwon, Kee-Yeon Kum, Woo-Cheol Lee, Seung-Ho Baek, Mo K. Kang, and Won-Jun Shon

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents