Early Steps of a Thymic Tumor in SV40 Transgenic Mice: Hyperplasia of Medullary Epithelial Cells and Increased Mature Thymocyte Numbers Disturb Thymic ExportReport as inadecuate




Early Steps of a Thymic Tumor in SV40 Transgenic Mice: Hyperplasia of Medullary Epithelial Cells and Increased Mature Thymocyte Numbers Disturb Thymic Export - Download this document for free, or read online. Document in PDF available to download.

Developmental Immunology - Volume 9 2002, Issue 4, Pages 223-231



INSERM U.345, Institut Necker, Paris Cedex 15 75730, France

INSERM U.238, CEA., Grenoble Cedex 9 38054, France

INSERM U.129, CHU Cochin-Port-Royal, Paris 75014, France



Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bone marrow progenitors migrate to the thymus, where they proliferate and differentiate into immunologically competent T cells. In this report we show that mice transgenic for SV40 T and t antigens under the control of the L-pyruvate kinase promoter develop, in a first step, thymic hyperplasia of both thymocytes and epithelial cells. Morphological studies histology, immunohistolabeling and electron microscopy revealed modifications of the thymic microenvironment and gradual expansion of medullary epithelial cells in 1 month-old mice, taking over the cortical region. Then, a thymic carcinoma develops. Two-color labeling of frozen sections identified the transgene in medullary epithelial cells. Flow cytometry analysis demonstrated a marked increase in mature CD4

and CD8

thymocytes in adult mice 39±10×10

in transgenic mice and 12±5×10

in age-matched controls. Furthermore, thymocyte export was disturbed.





Author: Bernadette Nabarra, Catherine Martinon, Cécile Godard, Florence Vasseur, Geoffroy de Ribains, Lucile Miquerol, Axel Kahn, and So

Source: https://www.hindawi.com/



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